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embryo fibroblast cell line  (ATCC)


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    ATCC embryo fibroblast cell line
    (A-B) <t>Fibroblast</t> GPL cells. (A) GPCMV(PC+) growth (MOI 1 pfu/cell, 3 DPI) on PDGFRA/NRP2 DKO GPL cells (black) compared to DKO cells ectopically expressing either NRP2 (green) or ThBD (red). Control wild type GPL(blue). One-way ANOVA p < 0.05. (B) GPCMV(PC+) growth on PDGFRA/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Both cell lines had minimal support for GPCMV infection. Student t test, ns = not significant). (C-D) Epithelial REPI cells. (D) GPCMV(PC+) growth (MOI 1 pfu/cell, 3DPI) on ThBD/NRP2 DKO REPI cells or DKO cells ectopically expressing NRP2 (green) or ThBD (red). Control wild type REPI (blue). One-way ANOVA p < 0.05. (D) GPCMV(PC+) growth on ThBD/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Student t test, ns = not significant).
    Embryo Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 105 article reviews
    embryo fibroblast cell line - by Bioz Stars, 2026-05
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    1) Product Images from "Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model"

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model

    Journal: bioRxiv

    doi: 10.64898/2026.05.07.723586

    (A-B) Fibroblast GPL cells. (A) GPCMV(PC+) growth (MOI 1 pfu/cell, 3 DPI) on PDGFRA/NRP2 DKO GPL cells (black) compared to DKO cells ectopically expressing either NRP2 (green) or ThBD (red). Control wild type GPL(blue). One-way ANOVA p < 0.05. (B) GPCMV(PC+) growth on PDGFRA/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Both cell lines had minimal support for GPCMV infection. Student t test, ns = not significant). (C-D) Epithelial REPI cells. (D) GPCMV(PC+) growth (MOI 1 pfu/cell, 3DPI) on ThBD/NRP2 DKO REPI cells or DKO cells ectopically expressing NRP2 (green) or ThBD (red). Control wild type REPI (blue). One-way ANOVA p < 0.05. (D) GPCMV(PC+) growth on ThBD/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Student t test, ns = not significant).
    Figure Legend Snippet: (A-B) Fibroblast GPL cells. (A) GPCMV(PC+) growth (MOI 1 pfu/cell, 3 DPI) on PDGFRA/NRP2 DKO GPL cells (black) compared to DKO cells ectopically expressing either NRP2 (green) or ThBD (red). Control wild type GPL(blue). One-way ANOVA p < 0.05. (B) GPCMV(PC+) growth on PDGFRA/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Both cell lines had minimal support for GPCMV infection. Student t test, ns = not significant). (C-D) Epithelial REPI cells. (D) GPCMV(PC+) growth (MOI 1 pfu/cell, 3DPI) on ThBD/NRP2 DKO REPI cells or DKO cells ectopically expressing NRP2 (green) or ThBD (red). Control wild type REPI (blue). One-way ANOVA p < 0.05. (D) GPCMV(PC+) growth on ThBD/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Student t test, ns = not significant).

    Techniques Used: Expressing, Control, Infection

    Wild type GPCMV virus stock generated on REPI cells (REPI stock) or by single passage on fibroblast GPL cells (GPL stock) were evaluated for ability to infect GPL fibroblasts, REPI and trophoblast (TEPI) epithelial cells. (A) Comparative one-step growth curve of GPCMV (MOI 1 pfu/cell) on GPL and PDGFRA KO GPL cells. Viral titers evaluated for 1-6 days post infection (dpi). GPCMV GPL stock (triangle), GPL cell infection (blue), PDGFRA KO GPL cells (black). GPCMV REPI stock (square), GPL cell infection (green), PDGFRA KO GPL cells (green). (B) Comparative one-step growth curve of GPCMV on REPI or TEPI cells by virus stock generated on GPL cells (triangle) or REPI cells (square). Experimental set up similar to (A) with MOI of 1 pfu/cell. GPCMV (GPL stock) infection of REPI cells (green triangle), or TEPI cells (blue triangle). GPCMV (REPI stock) infection of REPI cells (grey square), or TEPI cells (purple square). Viral titers (1-6 dpi) were determined on GPL cells as described for A. Results shown are average values from replicates carried out in triplicate.
    Figure Legend Snippet: Wild type GPCMV virus stock generated on REPI cells (REPI stock) or by single passage on fibroblast GPL cells (GPL stock) were evaluated for ability to infect GPL fibroblasts, REPI and trophoblast (TEPI) epithelial cells. (A) Comparative one-step growth curve of GPCMV (MOI 1 pfu/cell) on GPL and PDGFRA KO GPL cells. Viral titers evaluated for 1-6 days post infection (dpi). GPCMV GPL stock (triangle), GPL cell infection (blue), PDGFRA KO GPL cells (black). GPCMV REPI stock (square), GPL cell infection (green), PDGFRA KO GPL cells (green). (B) Comparative one-step growth curve of GPCMV on REPI or TEPI cells by virus stock generated on GPL cells (triangle) or REPI cells (square). Experimental set up similar to (A) with MOI of 1 pfu/cell. GPCMV (GPL stock) infection of REPI cells (green triangle), or TEPI cells (blue triangle). GPCMV (REPI stock) infection of REPI cells (grey square), or TEPI cells (purple square). Viral titers (1-6 dpi) were determined on GPL cells as described for A. Results shown are average values from replicates carried out in triplicate.

    Techniques Used: Virus, Generated, Infection



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    Image Search Results


    (A-B) Fibroblast GPL cells. (A) GPCMV(PC+) growth (MOI 1 pfu/cell, 3 DPI) on PDGFRA/NRP2 DKO GPL cells (black) compared to DKO cells ectopically expressing either NRP2 (green) or ThBD (red). Control wild type GPL(blue). One-way ANOVA p < 0.05. (B) GPCMV(PC+) growth on PDGFRA/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Both cell lines had minimal support for GPCMV infection. Student t test, ns = not significant). (C-D) Epithelial REPI cells. (D) GPCMV(PC+) growth (MOI 1 pfu/cell, 3DPI) on ThBD/NRP2 DKO REPI cells or DKO cells ectopically expressing NRP2 (green) or ThBD (red). Control wild type REPI (blue). One-way ANOVA p < 0.05. (D) GPCMV(PC+) growth on ThBD/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Student t test, ns = not significant).

    Journal: bioRxiv

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model

    doi: 10.64898/2026.05.07.723586

    Figure Lengend Snippet: (A-B) Fibroblast GPL cells. (A) GPCMV(PC+) growth (MOI 1 pfu/cell, 3 DPI) on PDGFRA/NRP2 DKO GPL cells (black) compared to DKO cells ectopically expressing either NRP2 (green) or ThBD (red). Control wild type GPL(blue). One-way ANOVA p < 0.05. (B) GPCMV(PC+) growth on PDGFRA/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Both cell lines had minimal support for GPCMV infection. Student t test, ns = not significant). (C-D) Epithelial REPI cells. (D) GPCMV(PC+) growth (MOI 1 pfu/cell, 3DPI) on ThBD/NRP2 DKO REPI cells or DKO cells ectopically expressing NRP2 (green) or ThBD (red). Control wild type REPI (blue). One-way ANOVA p < 0.05. (D) GPCMV(PC+) growth on ThBD/NRP2 DKO GPL (black) compared to PDGFRA/NRP2 DKO GPL + ectopic expression of CD46 (orange). Student t test, ns = not significant).

    Article Snippet: However, studies from the mid-1990s onwards utilized an established ATCC embryo fibroblast cell line (GPL ATCC CCL 158) for virus growth and titration experiments because of an ability of cells to be contact inhibited as a confluent monolayer and these cells easily supports virus, BAC and plasmid transfections ( ).

    Techniques: Expressing, Control, Infection

    Wild type GPCMV virus stock generated on REPI cells (REPI stock) or by single passage on fibroblast GPL cells (GPL stock) were evaluated for ability to infect GPL fibroblasts, REPI and trophoblast (TEPI) epithelial cells. (A) Comparative one-step growth curve of GPCMV (MOI 1 pfu/cell) on GPL and PDGFRA KO GPL cells. Viral titers evaluated for 1-6 days post infection (dpi). GPCMV GPL stock (triangle), GPL cell infection (blue), PDGFRA KO GPL cells (black). GPCMV REPI stock (square), GPL cell infection (green), PDGFRA KO GPL cells (green). (B) Comparative one-step growth curve of GPCMV on REPI or TEPI cells by virus stock generated on GPL cells (triangle) or REPI cells (square). Experimental set up similar to (A) with MOI of 1 pfu/cell. GPCMV (GPL stock) infection of REPI cells (green triangle), or TEPI cells (blue triangle). GPCMV (REPI stock) infection of REPI cells (grey square), or TEPI cells (purple square). Viral titers (1-6 dpi) were determined on GPL cells as described for A. Results shown are average values from replicates carried out in triplicate.

    Journal: bioRxiv

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model

    doi: 10.64898/2026.05.07.723586

    Figure Lengend Snippet: Wild type GPCMV virus stock generated on REPI cells (REPI stock) or by single passage on fibroblast GPL cells (GPL stock) were evaluated for ability to infect GPL fibroblasts, REPI and trophoblast (TEPI) epithelial cells. (A) Comparative one-step growth curve of GPCMV (MOI 1 pfu/cell) on GPL and PDGFRA KO GPL cells. Viral titers evaluated for 1-6 days post infection (dpi). GPCMV GPL stock (triangle), GPL cell infection (blue), PDGFRA KO GPL cells (black). GPCMV REPI stock (square), GPL cell infection (green), PDGFRA KO GPL cells (green). (B) Comparative one-step growth curve of GPCMV on REPI or TEPI cells by virus stock generated on GPL cells (triangle) or REPI cells (square). Experimental set up similar to (A) with MOI of 1 pfu/cell. GPCMV (GPL stock) infection of REPI cells (green triangle), or TEPI cells (blue triangle). GPCMV (REPI stock) infection of REPI cells (grey square), or TEPI cells (purple square). Viral titers (1-6 dpi) were determined on GPL cells as described for A. Results shown are average values from replicates carried out in triplicate.

    Article Snippet: However, studies from the mid-1990s onwards utilized an established ATCC embryo fibroblast cell line (GPL ATCC CCL 158) for virus growth and titration experiments because of an ability of cells to be contact inhibited as a confluent monolayer and these cells easily supports virus, BAC and plasmid transfections ( ).

    Techniques: Virus, Generated, Infection